铔嬬櫧纾烽吀鍖栫爺绌剁殑棰勫埗鑳迭img src="http://labchem.fujifilm-wako.com.cn/upfiles/images/201701/2214850785628047.jpg" title="2214850785628047.jpg" alt="鏂板搧閫熼€掑浘wako.jpg" style="margin: 0px; padding: 0px; font-family: Arial, 寰蒋闆呴粦, sans-serif; border: none; float: right;"/>
SuperSep Phos-tag鈩↑/span>
銆€銆€SuperSep Phos-tag鈩 鏄爺绌惰泲鐧界７閰稿寲鐨勬柟娉曪紝鏃犻渶鐗瑰紓鎬х７閰稿寲鎶椾綋鎴栬€呭悓浣嶇礌鏍囪銆侟/span>
鈼咟/span>SuperSep Phos-tag鈩↑/span>
銆€銆€Phos-tag鈩 鏄竴绉嶉鍒惰兌锛岄鍏堝姞鍏ヤ簡 50 渭mol/L 鐨 Phostag鈩 Acrylamide锛屾墦寮€鍖呰鍗冲彲鐩存帴浣跨敤銆傞鍒惰兌涓惈鏈夐攲浣滀负閲戝睘绂诲瓙锛屽湪涓績鍑濊兌缂撳啿娑蹭腑淇濆瓨绋冲畾鎬у緢濂斤紝寰楀埌鐨勫甫鏉＄粨鏋滀篃寰堟暣榻愩€侟/span>
銆€銆€纾烽吀鍖栬泲鐧藉拰闈炵７閰稿寲铔嬬櫧浣滀负涓嶅悓鏉″甫鍒嗙銆侟/span>
銆€銆€鍒嗙鍚庯紝鑳跺彲鐢ㄤ簬鑰冮┈鏂寒钃濇煋鑹诧紝鍏嶇柅鍗拌抗鍜岃川璋卞疄楠屻€侟/span>
宸插叕寮€鐨勯獙璇佽泲鐧藉垪琛紝璇风偣鍑狐/strong>
鈼咟/span>Phos-tag鈩 SDS-PAGE 鐨勫師鐞咟/span>
鈼咟/span>鍦 HighWire Search 涓婃悳绱㈠埌鐨勮鏂囨暟
鈼咟/span>杩愮敤
鍒╃敤 p35 鐨勪笝姘ㄩ吀绐佸彉浣撶‘瀹 Cdk5 婵€娲 p35 鐨勭７閰稿寲浣嶇偣
銆€銆€p35 甯歌鐨勭７閰稿寲浣嶇偣鏄 Ser8 鍜 Thr138銆備絾鏄 Ser8 鍜 Thr138 浣嶇偣寰€寰€浼氬彂鐢熶笝姘ㄩ吀绐佸彉锛屼骇鐢燂紦绉嶇獊鍙樹綋锛圫er8 绐佸彉浣擄細S8A锛孴hr138 绐佸彉浣擄細T138A锛孲er8 鍜 Thr138 鍙岀獊鍙樹綋锛?A锛夈€傝繖锛撶绐佸彉浣撱€侀噹鐢熷瀷 p35銆丆dk5 鍜屾病鏈夋縺閰舵椿鎬х殑 Cdk5 閮芥潵婧愪簬 COS-7 缁嗚優銆傝繖浜涚粏鑳炶瑙ｆ恫鐢?/span>Phos-tag鈩 SDS-PAGE 鍜 Western blotting 杩涜妫€娴嬶紙妫€娴嬫姉浣擄細p35 鎶椾綋锛夈€侟/span>
100 渭M Phos-tag 鈩 涓欑儻閰拌兒, 7.5% 鑱氫笝鐑叞鑳哄嚌鑳迭/span>
鍙槑纭７閰稿寲浣嶇偣鍜屾潯甯﹁縼绉荤巼鐨勫叧绯伙紒
- 娉抽亾锛戯紙鏉″甫 L2 鍜 L4锛夊拰娉抽亾锛曪紙鏉″甫 M1锛夛細p35 鍦 Cdk5 鐨勪綔鐢ㄤ笅鍙戠敓浜嗙７閰稿寲锛汓/span>
- 娉抽亾锛戯紙鏉″甫 L2 鍜 L4锛夊拰娉抽亾锛擄紙鏉″甫 L2 鍜 L4锛夛細鍦ㄦ棤婵€閰舵椿鎬 Cdk5 鐨勪綔鐢ㄤ笅锛屽ぇ绾︽湁涓€鍗 p35 铔嬬櫧鍦 Thr138 浣嶇偣鍙戠敓纾烽吀鍖栵紝鍚屾牱鍦 138 浣嶅彂鐢熺獊鍙樼殑 p35 铔嬬櫧浜︽槸濡傛銆侟/span>
- 娉抽亾锛 锛堟潯甯 M1锛夊拰娉抽亾锛栵紙鏉″甫 L3 鍜 L4锛夛細Ser8 鍜 Thr138 鏄富瑕佺殑纾烽吀鍖栦綅鐐癸紱
- 娉抽亾锛曪紙鏉″甫 M1锛夈€佹吵閬擄紬锛堟潯甯1鍜孡2锛夊拰娉抽亾锛橈紙鏉″甫M2锛夛細鏉″甫 M1 鏄 Ser8 鍜孴 hr138 閮藉彂鐢熺７閰稿寲鐨勬潯甯︼紱
鏉″甫 M2 鏄彧鏈 Ser8 纾烽吀鍖栫殑鏉″甫锛涙潯甯 L1 鍜 L2 鏄彧鏈 Thr138 纾烽吀鍖栫殑鏉″甫銆侟/span>
鈥 鏉″甫 L1 鍜 L3 涓殑锛告槸涓嶇‘瀹氬摢涓綅鐐瑰彂鐢熺７閰稿寲鐨勬潯甯︼紱
鈥?nbsp;鏉″甫L4鏄潪纾烽吀鍖栫殑 p35 銆侟/span>
銆愬弬鑰冩枃鐚€慄/span>
鈻 Quantitative Measurement of in Vivo Phosphorylation States of Cdk5 Activator p35 by Phos-tag 鈩 SDS-PAGE. T. Hosokawa, T. Saito, A. Asada, K. Fukunaga, and S. Hisanaga,Mol. Cell. Proteomics, Jun 2010;9: 1133 - 1143.
銆愮粨鏋滄彁渚涖€慄/span>
鐞嗗寲瀛︾爺绌舵墍 鑴戠瀛︾患鍚堢爺绌朵腑蹇 鍥炶矾鍔熻兘鐮旂┒鏍稿績 璁板繂鍔熻兘鐮旂┒鍥㈤槦 缁嗗窛鏅烘案锛圖r. T. Hosokawa锛堻/span>
棣栭兘澶у涓滀含 鐞嗗伐瀛︾爺绌剁 鐢熷懡绉戝涓撲笟 绁炵粡鍒嗗瓙鍔熻兘鐮旂┒瀹 涔呮案鐪熷競锛圖r. S. Hisanaga锛堻/span>
妫€娴嬪惈鏈 Dnmt1 纾烽吀鍖栨縺閰剁殑鐗囨
鎴戜滑鍙互纭畾鍦ㄧ墖娈典腑鍚湁鐩殑婵€閰讹紒
鈶?nbsp;閲囩敤浜插拰鑹茶氨娉曚粠榧犺剳鎻愬彇娑蹭腑绾寲 GST-Dnmt1锛?-290锛夌粨鍚堣泲鐧键/span>
鈶 浣跨敤 0.3 M 鍜 1 M NaCl 鐨 DNA 绾ょ淮绱犳煴娲楄劚寰楀埌鐩殑铔嬬櫧
鈶 GST-Dnmt1锛?-290锛変綔涓轰綋澶栨縺閰跺疄楠岀殑鍙嶅簲搴曠墿
鈶 Phos-tag 鈩 SDS-PAGE 鐢ㄤ簬Western blotting锛岀‘瀹氳縼绉绘潯甯︿腑姣忎釜鐗囨鐨勬縺閰舵椿鎬?/span>
銆愬弬鑰冩枃鐚€慄/span>
The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1delta/epsilon. Y. Sugiyama, N. Hatano, N. Sueyoshi, I. Suetake, S. Tajima, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, and I. Kameshita, Biochem. J., May 2010; 427(3): 489-97.
銆愮粨鏋滄彁渚涖€慄/span>
楂樼煡澶у 缁煎悎鐮旂┒涓績 鐢熷懡銆佸姛鑳界墿璐ㄩ儴闂 瀹為獙瀹炰範鏈哄櫒璁炬柦 鏉夊北搴峰锛圖r. Y. Sugiyama锛堻/span>
棣欏窛澶у 鍐滃閮 搴旂敤鐢熺墿绉戝绉 鍔ㄧ墿鍔熻兘鐢熷寲瀛︾爺绌跺 榫熶笅鍕囷紙Dr. I. Kameshita锛堻/span>
浜岀淮鐢垫吵涓殑搴旂敤锛氬垎鏋 hnRNP K 纾烽吀鍖栧紓鏋勪綋
銆€銆€灏忛紶宸ㄥ櫖缁嗚優 J774.1 缁 LPS 鍒烘縺鍚庯紝瑁傝В缁嗚優锛岀粡杩囧厤鐤矇娣€娉曞垎绂诲緱鍒 hnRNP K銆傚湪浜岀淮鐢垫吵涓紝涓€缁存槸 IPG 鑳讹紝浜岀淮鏄 Phos-tag 鈩 SDS-PAGE锛屽彲鍒嗙 hnRNP K 鐨勫紓鏋勪綋銆傚埄鐢ㄨ川璋变华锛屽彲浠ョ‘璁や笉鍚岀殑鐐逛唬琛ㄤ笉鍚岀殑浜氬瀷鎴栦慨楗拌泲鐧姐€侟/span>
鍚屼竴涓瓑鐢电偣鐨勪綅缃笂锛屼笉鍚屼綅鐐瑰彂鐢熺７閰稿寲閮藉彲浠ヨ鍖哄垎寮€鏉?/span>
锛堜緥: spots 6 vs. 8 and spots 4 vs. 7锛堻/span>
銆愬弬鑰冩枃鐚€慄/span>
鈻 Characterization of multiple alternative forms of heterogeneous nuclear ribonucleoprotein K by phosphate-affinity electrophoresis. Y. Kimura, K. Nagata, N Suzuki, R. Yokoyama, Y. Yamanaka, H. Kitamura, H. Hirano, and O. Ohara, Proteomics, Nov 2010; 10(21): 3884-95.
銆愮粨鏋滄彁渚涖€慄/span>
妯花甯傜珛澶у 鐢熷懡绾崇背绯荤粺绉戝鐮旂┒绉 鐢熺墿浣撹秴鍒嗗瓙绯荤粺绉戝涓撲笟 鏈ㄦ潙寮ョ敓锛圖r. Y. Kimura锛夈€佸钩閲庝箙锛圖r. H. Hirano锛堻/span>
鐞嗗寲瀛︾爺绌舵墍 RCAI 灏忓師鏀迭/span>
鈼咟/span>澶囨敞
鏍峰搧鍒跺锛欬/span>
Phos-tag SDS-PAGE 瀵逛簬铔嬬櫧鏍峰搧涓殑鏉傝川闈炲父鏁忔劅锛屽挨鍏舵槸铻悎鍓傦紝閽掗吀锛屾棤鏈虹洂锛岃〃闈㈡椿鎬у墏杩欑被鐗╄川銆侟/span>
寮虹儓寤鸿鍦 Phos-tag SDS-PAGE 涔嬪墠閫氳繃 TCA 娌夋穩鎴栨笚鏋愭硶闄嶄綆鏉傝川鍚噺銆侟/span>
杞啘鍓嶅鐞嗭細
鍙︿竴涓繀椤荤殑姝ラ鏄湪杞啘鍓嶏紝鐢 EDTA 鍘婚櫎鍑濊兌涓殑閲戝睘绂诲瓙锛圡n2+ 鎴栬€匷n2+锛夛紱璇ユ楠ゅ彲鎻愰珮铔嬬櫧鐨勮浆鑶滄晥鐜囥€侟/span>
鈼忋€€鍒嗗埆鍑嗗 10 mmol/L 鍚 EDTA 鍜屼笉鍚 EDTA 涓ょ 1x transfer buffer銆侟/span>
鈼忋€€灏嗗嚌鑳舵蹈娉″湪鍚 10 mmol/L EDTA 鐨 1x transfer buffer锛岃嚦灏 20 鍒嗛挓锛屾俯鍜屾憞鏅冦€傛洿鎹㈡柊缂撳啿娑诧紝閲嶅锛撴銆侟/span>
鈼忋€€灏嗗嚌鑳舵蹈娉″湪涓嶅惈 10 mmol/L EDTA 鐨 1x transfer buffer锛?0 鍒嗛挓锛屾俯鍜屾憞鏅冦€侟/span>
鈼忋€€杞啘鎿嶄綔*銆侟/span>
* 寤鸿鐢ㄦ箍娉曡浆鑶滐紝浠ユ彁楂樿浆鑶滄晥鐜囥€侟/span>
鈼咟/span>璐ㄩ噺鎺у埗
銆€銆€姣忎竴鎵 SuperSep Phos-tag鈩紝鍑哄巶鍓嶅潎鏍规嵁鍏朵骇鍝佽鏍艰繘琛屾祴璇曪紝浠ヤ繚璇佸彲鍒嗙纾烽吀鍖栧拰闈炵７閰稿寲铔嬬櫧锛屼互鍙婁粬浠殑鍒嗙鎴愰兘鍦ㄦ甯稿弬鏁板唴銆侟/span>
鈼嗕繚瀛樻俯搴?/strong>
2-10鈩傸/span>
鈼嗕骇鍝佷俊鎭?/span>
鐢ㄤ簬 Bio-Rad 浼箰鐢垫吵浠?/span>
璐у彿 | 鍝佸悕 | 鐢垫吵浠?/span> | 瑙勬牸 |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 7.5%, 17well, 83脳100脳3.9mm | Mini-PROTEAN庐 Tetra Cell (Bio-Rad Laboratories, Inc.) | 5 鍧桙/span> | |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 12.5%, 17well, 83脳100脳3.9mm | 5 鍧桙/span> |
鐢ㄤ簬 Life Technologies 鐢垫吵浠?/span>
璐у彿 | 鍝佸悕 | 鐢垫吵浠?/span> | 瑙勬牸 |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 7.5%, 17well, 100脳100脳6.6mm | XCell SureLock庐 Mini-Cell (Life Technologies, Inc.) | 5 鍧桙/span> | |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 12.5%, 17well, 100脳100脳6.6mm | 5 鍧桙br style="margin: 0px; padding: 0px; font-family: Arial, 寰蒋闆呴粦, sans-serif;"/> |
鐢ㄤ簬 Wako EasySeperator 鐢垫吵浠?/span>
| 浜у搧缂栧彿 | 浜у搧 | 鍑濊兌娴撳害 | 瀛旀暟 | 鍖呰 |
| 192-17401 | SuperSep鈩 Phos-tag鈩 (50 渭mol/L) Phos-tag鈩 棰勫埗鑳?0 渭mol/L | 6.0% | 13瀛擖/span> | 5鍧桙/span> |
| 199-17391 | 6.0% | 17瀛擖/span> | 5鍧桙/span> | |
| 195-17371 | 7.5% | 13瀛擖/span> | 5鍧桙/span> | |
| 192-17381 | 7.5% | 17瀛擖/span> | 5鍧桙/span> | |
| 193-16711 | 10.0% | 13瀛擖/span> | 5鍧桙/span> | |
| 190-16721 | 10.0% | 17瀛擖/span> | 5鍧桙/span> | |
| 195-16391 | 12.5% | 13瀛擖/span> | 5鍧桙/span> | |
| 193-16571 | 12.5% | 17瀛擖/span> | 5鍧桙/span> | |
| 193-16691 | 15.0% | 13瀛擖/span> | 5鍧桙/span> | |
| 196-16701 | 15.0% | 17瀛擖/span> | 5鍧桙/span> | |
| 197-16851 | 17.5% | 13瀛擖/span> | 5鍧桙/span> | |
| 194-16861 | 17.5% | 17瀛擖/span> | 5鍧桙/span> |
鈼嗙浉鍏充骇鍝?/strong>
| 浜у搧缂栧彿 | 浜у搧鍚嶇О | 瑙勬牸 |
| 058-07681 | EasySeparator Phos-tag棰勫埗鍑濊兌鐨勯厤濂楃數娉虫Ы | 1 set |
缁嗚優鍒嗘瀽
缁嗚優娌荤枟
澶栨硨浣撶爺绌迭/a>
鐢熸畺宸ョ▼
鏂拌嵂鐮斿彂&鐢熺墿宸ヨ壓
缁嗚優鍩瑰吇
鐤剧梾鐮旂┒
铔嬬櫧鐮旂┒
杞煋
鑽墿绛涢€堻/a>
鑽悊姣掔悊
骞茬粏鑳炵爺绌迭/a>
琛ㄨ閬椾紶瀛?/a>
鍒嗗瓙鐢熺墿瀛?/a>
妞嶇墿瀛?/a>
寰敓鐗╁
鑰楁潗
鐢熺墿娲绘€х墿璐?/a>
鍏朵粬
浜у搧涓績 > 鐢熷懡绉戝 > 铔嬬櫧鐮旂┒ > 纾烽吀鍖栬泲鐧键/a>
SuperSep Phos-tag鈩 棰勫埗鑳迭br style="margin: 0px; padding: 0px;"/>SuperSep Phos-tag鈩↑/p>
铔嬬櫧纾烽吀鍖栫爺绌剁殑棰勫埗鑳迭img src="http://labchem.fujifilm-wako.com.cn/upfiles/images/201701/2214850785628047.jpg" title="2214850785628047.jpg" alt="鏂板搧閫熼€掑浘wako.jpg" style="margin: 0px; padding: 0px; font-family: Arial, 寰蒋闆呴粦, sans-serif; border: none; float: right;"/>
SuperSep Phos-tag鈩↑/span>
銆€銆€SuperSep Phos-tag鈩 鏄爺绌惰泲鐧界７閰稿寲鐨勬柟娉曪紝鏃犻渶鐗瑰紓鎬х７閰稿寲鎶椾綋鎴栬€呭悓浣嶇礌鏍囪銆侟/span>
鈼咟/span>SuperSep Phos-tag鈩↑/span>
銆€銆€Phos-tag鈩 鏄竴绉嶉鍒惰兌锛岄鍏堝姞鍏ヤ簡 50 渭mol/L 鐨 Phostag鈩 Acrylamide锛屾墦寮€鍖呰鍗冲彲鐩存帴浣跨敤銆傞鍒惰兌涓惈鏈夐攲浣滀负閲戝睘绂诲瓙锛屽湪涓績鍑濊兌缂撳啿娑蹭腑淇濆瓨绋冲畾鎬у緢濂斤紝寰楀埌鐨勫甫鏉＄粨鏋滀篃寰堟暣榻愩€侟/span>
銆€銆€纾烽吀鍖栬泲鐧藉拰闈炵７閰稿寲铔嬬櫧浣滀负涓嶅悓鏉″甫鍒嗙銆侟/span>
銆€銆€鍒嗙鍚庯紝鑳跺彲鐢ㄤ簬鑰冮┈鏂寒钃濇煋鑹诧紝鍏嶇柅鍗拌抗鍜岃川璋卞疄楠屻€侟/span>
宸插叕寮€鐨勯獙璇佽泲鐧藉垪琛紝璇风偣鍑狐/strong>
鈼咟/span>Phos-tag鈩 SDS-PAGE 鐨勫師鐞咟/span>
鈼咟/span>鍦 HighWire Search 涓婃悳绱㈠埌鐨勮鏂囨暟
鈼咟/span>杩愮敤
鍒╃敤 p35 鐨勪笝姘ㄩ吀绐佸彉浣撶‘瀹 Cdk5 婵€娲 p35 鐨勭７閰稿寲浣嶇偣
銆€銆€p35 甯歌鐨勭７閰稿寲浣嶇偣鏄 Ser8 鍜 Thr138銆備絾鏄 Ser8 鍜 Thr138 浣嶇偣寰€寰€浼氬彂鐢熶笝姘ㄩ吀绐佸彉锛屼骇鐢燂紦绉嶇獊鍙樹綋锛圫er8 绐佸彉浣擄細S8A锛孴hr138 绐佸彉浣擄細T138A锛孲er8 鍜 Thr138 鍙岀獊鍙樹綋锛?A锛夈€傝繖锛撶绐佸彉浣撱€侀噹鐢熷瀷 p35銆丆dk5 鍜屾病鏈夋縺閰舵椿鎬х殑 Cdk5 閮芥潵婧愪簬 COS-7 缁嗚優銆傝繖浜涚粏鑳炶瑙ｆ恫鐢?/span>Phos-tag鈩 SDS-PAGE 鍜 Western blotting 杩涜妫€娴嬶紙妫€娴嬫姉浣擄細p35 鎶椾綋锛夈€侟/span>
100 渭M Phos-tag 鈩 涓欑儻閰拌兒, 7.5% 鑱氫笝鐑叞鑳哄嚌鑳迭/span>
鍙槑纭７閰稿寲浣嶇偣鍜屾潯甯﹁縼绉荤巼鐨勫叧绯伙紒
- 娉抽亾锛戯紙鏉″甫 L2 鍜 L4锛夊拰娉抽亾锛曪紙鏉″甫 M1锛夛細p35 鍦 Cdk5 鐨勪綔鐢ㄤ笅鍙戠敓浜嗙７閰稿寲锛汓/span>
- 娉抽亾锛戯紙鏉″甫 L2 鍜 L4锛夊拰娉抽亾锛擄紙鏉″甫 L2 鍜 L4锛夛細鍦ㄦ棤婵€閰舵椿鎬 Cdk5 鐨勪綔鐢ㄤ笅锛屽ぇ绾︽湁涓€鍗 p35 铔嬬櫧鍦 Thr138 浣嶇偣鍙戠敓纾烽吀鍖栵紝鍚屾牱鍦 138 浣嶅彂鐢熺獊鍙樼殑 p35 铔嬬櫧浜︽槸濡傛銆侟/span>
- 娉抽亾锛 锛堟潯甯 M1锛夊拰娉抽亾锛栵紙鏉″甫 L3 鍜 L4锛夛細Ser8 鍜 Thr138 鏄富瑕佺殑纾烽吀鍖栦綅鐐癸紱
- 娉抽亾锛曪紙鏉″甫 M1锛夈€佹吵閬擄紬锛堟潯甯1鍜孡2锛夊拰娉抽亾锛橈紙鏉″甫M2锛夛細鏉″甫 M1 鏄 Ser8 鍜孴 hr138 閮藉彂鐢熺７閰稿寲鐨勬潯甯︼紱
鏉″甫 M2 鏄彧鏈 Ser8 纾烽吀鍖栫殑鏉″甫锛涙潯甯 L1 鍜 L2 鏄彧鏈 Thr138 纾烽吀鍖栫殑鏉″甫銆侟/span>
鈥 鏉″甫 L1 鍜 L3 涓殑锛告槸涓嶇‘瀹氬摢涓綅鐐瑰彂鐢熺７閰稿寲鐨勬潯甯︼紱
鈥?nbsp;鏉″甫L4鏄潪纾烽吀鍖栫殑 p35 銆侟/span>
銆愬弬鑰冩枃鐚€慄/span>
鈻 Quantitative Measurement of in Vivo Phosphorylation States of Cdk5 Activator p35 by Phos-tag 鈩 SDS-PAGE. T. Hosokawa, T. Saito, A. Asada, K. Fukunaga, and S. Hisanaga,Mol. Cell. Proteomics, Jun 2010;9: 1133 - 1143.
銆愮粨鏋滄彁渚涖€慄/span>
鐞嗗寲瀛︾爺绌舵墍 鑴戠瀛︾患鍚堢爺绌朵腑蹇 鍥炶矾鍔熻兘鐮旂┒鏍稿績 璁板繂鍔熻兘鐮旂┒鍥㈤槦 缁嗗窛鏅烘案锛圖r. T. Hosokawa锛堻/span>
棣栭兘澶у涓滀含 鐞嗗伐瀛︾爺绌剁 鐢熷懡绉戝涓撲笟 绁炵粡鍒嗗瓙鍔熻兘鐮旂┒瀹 涔呮案鐪熷競锛圖r. S. Hisanaga锛堻/span>
妫€娴嬪惈鏈 Dnmt1 纾烽吀鍖栨縺閰剁殑鐗囨
鎴戜滑鍙互纭畾鍦ㄧ墖娈典腑鍚湁鐩殑婵€閰讹紒
鈶?nbsp;閲囩敤浜插拰鑹茶氨娉曚粠榧犺剳鎻愬彇娑蹭腑绾寲 GST-Dnmt1锛?-290锛夌粨鍚堣泲鐧键/span>
鈶 浣跨敤 0.3 M 鍜 1 M NaCl 鐨 DNA 绾ょ淮绱犳煴娲楄劚寰楀埌鐩殑铔嬬櫧
鈶 GST-Dnmt1锛?-290锛変綔涓轰綋澶栨縺閰跺疄楠岀殑鍙嶅簲搴曠墿
鈶 Phos-tag 鈩 SDS-PAGE 鐢ㄤ簬Western blotting锛岀‘瀹氳縼绉绘潯甯︿腑姣忎釜鐗囨鐨勬縺閰舵椿鎬?/span>
銆愬弬鑰冩枃鐚€慄/span>
The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1delta/epsilon. Y. Sugiyama, N. Hatano, N. Sueyoshi, I. Suetake, S. Tajima, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, and I. Kameshita, Biochem. J., May 2010; 427(3): 489-97.
銆愮粨鏋滄彁渚涖€慄/span>
楂樼煡澶у 缁煎悎鐮旂┒涓績 鐢熷懡銆佸姛鑳界墿璐ㄩ儴闂 瀹為獙瀹炰範鏈哄櫒璁炬柦 鏉夊北搴峰锛圖r. Y. Sugiyama锛堻/span>
棣欏窛澶у 鍐滃閮 搴旂敤鐢熺墿绉戝绉 鍔ㄧ墿鍔熻兘鐢熷寲瀛︾爺绌跺 榫熶笅鍕囷紙Dr. I. Kameshita锛堻/span>
浜岀淮鐢垫吵涓殑搴旂敤锛氬垎鏋 hnRNP K 纾烽吀鍖栧紓鏋勪綋
銆€銆€灏忛紶宸ㄥ櫖缁嗚優 J774.1 缁 LPS 鍒烘縺鍚庯紝瑁傝В缁嗚優锛岀粡杩囧厤鐤矇娣€娉曞垎绂诲緱鍒 hnRNP K銆傚湪浜岀淮鐢垫吵涓紝涓€缁存槸 IPG 鑳讹紝浜岀淮鏄 Phos-tag 鈩 SDS-PAGE锛屽彲鍒嗙 hnRNP K 鐨勫紓鏋勪綋銆傚埄鐢ㄨ川璋变华锛屽彲浠ョ‘璁や笉鍚岀殑鐐逛唬琛ㄤ笉鍚岀殑浜氬瀷鎴栦慨楗拌泲鐧姐€侟/span>
鍚屼竴涓瓑鐢电偣鐨勪綅缃笂锛屼笉鍚屼綅鐐瑰彂鐢熺７閰稿寲閮藉彲浠ヨ鍖哄垎寮€鏉?/span>
锛堜緥: spots 6 vs. 8 and spots 4 vs. 7锛堻/span>
銆愬弬鑰冩枃鐚€慄/span>
鈻 Characterization of multiple alternative forms of heterogeneous nuclear ribonucleoprotein K by phosphate-affinity electrophoresis. Y. Kimura, K. Nagata, N Suzuki, R. Yokoyama, Y. Yamanaka, H. Kitamura, H. Hirano, and O. Ohara, Proteomics, Nov 2010; 10(21): 3884-95.
銆愮粨鏋滄彁渚涖€慄/span>
妯花甯傜珛澶у 鐢熷懡绾崇背绯荤粺绉戝鐮旂┒绉 鐢熺墿浣撹秴鍒嗗瓙绯荤粺绉戝涓撲笟 鏈ㄦ潙寮ョ敓锛圖r. Y. Kimura锛夈€佸钩閲庝箙锛圖r. H. Hirano锛堻/span>
鐞嗗寲瀛︾爺绌舵墍 RCAI 灏忓師鏀迭/span>
鈼咟/span>澶囨敞
鏍峰搧鍒跺锛欬/span>
Phos-tag SDS-PAGE 瀵逛簬铔嬬櫧鏍峰搧涓殑鏉傝川闈炲父鏁忔劅锛屽挨鍏舵槸铻悎鍓傦紝閽掗吀锛屾棤鏈虹洂锛岃〃闈㈡椿鎬у墏杩欑被鐗╄川銆侟/span>
寮虹儓寤鸿鍦 Phos-tag SDS-PAGE 涔嬪墠閫氳繃 TCA 娌夋穩鎴栨笚鏋愭硶闄嶄綆鏉傝川鍚噺銆侟/span>
杞啘鍓嶅鐞嗭細
鍙︿竴涓繀椤荤殑姝ラ鏄湪杞啘鍓嶏紝鐢 EDTA 鍘婚櫎鍑濊兌涓殑閲戝睘绂诲瓙锛圡n2+ 鎴栬€匷n2+锛夛紱璇ユ楠ゅ彲鎻愰珮铔嬬櫧鐨勮浆鑶滄晥鐜囥€侟/span>
鈼忋€€鍒嗗埆鍑嗗 10 mmol/L 鍚 EDTA 鍜屼笉鍚 EDTA 涓ょ 1x transfer buffer銆侟/span>
鈼忋€€灏嗗嚌鑳舵蹈娉″湪鍚 10 mmol/L EDTA 鐨 1x transfer buffer锛岃嚦灏 20 鍒嗛挓锛屾俯鍜屾憞鏅冦€傛洿鎹㈡柊缂撳啿娑诧紝閲嶅锛撴銆侟/span>
鈼忋€€灏嗗嚌鑳舵蹈娉″湪涓嶅惈 10 mmol/L EDTA 鐨 1x transfer buffer锛?0 鍒嗛挓锛屾俯鍜屾憞鏅冦€侟/span>
鈼忋€€杞啘鎿嶄綔*銆侟/span>
* 寤鸿鐢ㄦ箍娉曡浆鑶滐紝浠ユ彁楂樿浆鑶滄晥鐜囥€侟/span>
鈼咟/span>璐ㄩ噺鎺у埗
銆€銆€姣忎竴鎵 SuperSep Phos-tag鈩紝鍑哄巶鍓嶅潎鏍规嵁鍏朵骇鍝佽鏍艰繘琛屾祴璇曪紝浠ヤ繚璇佸彲鍒嗙纾烽吀鍖栧拰闈炵７閰稿寲铔嬬櫧锛屼互鍙婁粬浠殑鍒嗙鎴愰兘鍦ㄦ甯稿弬鏁板唴銆侟/span>
鈼嗕繚瀛樻俯搴?/strong>
2-10鈩傸/span>
鈼嗕骇鍝佷俊鎭?/span>
鐢ㄤ簬 Bio-Rad 浼箰鐢垫吵浠?/span>
璐у彿 | 鍝佸悕 | 鐢垫吵浠?/span> | 瑙勬牸 |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 7.5%, 17well, 83脳100脳3.9mm | Mini-PROTEAN庐 Tetra Cell (Bio-Rad Laboratories, Inc.) | 5 鍧桙/span> | |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 12.5%, 17well, 83脳100脳3.9mm | 5 鍧桙/span> |
鐢ㄤ簬 Life Technologies 鐢垫吵浠?/span>
璐у彿 | 鍝佸悕 | 鐢垫吵浠?/span> | 瑙勬牸 |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 7.5%, 17well, 100脳100脳6.6mm | XCell SureLock庐 Mini-Cell (Life Technologies, Inc.) | 5 鍧桙/span> | |
SuperSep鈩 Phos-tag鈩 (50渭mol/L), 12.5%, 17well, 100脳100脳6.6mm | 5 鍧桙br style="margin: 0px; padding: 0px; font-family: Arial, 寰蒋闆呴粦, sans-serif;"/> |
鐢ㄤ簬 Wako EasySeperator 鐢垫吵浠?/span>
| 浜у搧缂栧彿 | 浜у搧 | 鍑濊兌娴撳害 | 瀛旀暟 | 鍖呰 |
| 192-17401 | SuperSep鈩 Phos-tag鈩 (50 渭mol/L) Phos-tag鈩 棰勫埗鑳?0 渭mol/L | 6.0% | 13瀛擖/span> | 5鍧桙/span> |
| 199-17391 | 6.0% | 17瀛擖/span> | 5鍧桙/span> | |
| 195-17371 | 7.5% | 13瀛擖/span> | 5鍧桙/span> | |
| 192-17381 | 7.5% | 17瀛擖/span> | 5鍧桙/span> | |
| 193-16711 | 10.0% | 13瀛擖/span> | 5鍧桙/span> | |
| 190-16721 | 10.0% | 17瀛擖/span> | 5鍧桙/span> | |
| 195-16391 | 12.5% | 13瀛擖/span> | 5鍧桙/span> | |
| 193-16571 | 12.5% | 17瀛擖/span> | 5鍧桙/span> | |
| 193-16691 | 15.0% | 13瀛擖/span> | 5鍧桙/span> | |
| 196-16701 | 15.0% | 17瀛擖/span> | 5鍧桙/span> | |
| 197-16851 | 17.5% | 13瀛擖/span> | 5鍧桙/span> | |
| 194-16861 | 17.5% | 17瀛擖/span> | 5鍧桙/span> |
鈼嗙浉鍏充骇鍝?/strong>
| 浜у搧缂栧彿 | 浜у搧鍚嶇О | 瑙勬牸 |
| 058-07681 | EasySeparator Phos-tag棰勫埗鍑濊兌鐨勯厤濂楃數娉虫Ы | 1 set |
| 浜у搧缂栧彿 | 浜у搧鍚嶇О | 浜у搧瑙勬牸 | 浜у搧绛夌骇 |
| 193-16711 | SuperSep Phos-tag鈩?nbsp; (50 渭mol/L), 10%, 13 well Phos-tag 13瀛?0%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
| 190-16721 | SuperSep Phos-tag鈩?nbsp;(50 渭mol/L), 10%, 17 well Phos-tag 17瀛?0%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
| 195-16391 | SuperSep Phos-tag鈩?nbsp;(50 渭mol/L), 12.5%, 13 well Phos-tag 13瀛?2.5%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
| 193-16571 | SuperSep Phos-tag鈩?nbsp;(50 渭mol/L), 12.5%, 17 well Phos-tag 17瀛?2.5%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
| 193-16691 | SuperSep Phos-tag鈩?nbsp;(50 渭mol/L), 15%, 13 well Phos-tag 13瀛?5%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
| 196-16701 | SuperSep Phos-tag鈩?nbsp;(50 渭mol/L), 15%, 17 well Phos-tag 17瀛?5%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
| 197-16851 | SuperSep Phos-tag鈩?nbsp;(50 渭mol/L), 17.5%, 13 well Phos-tag 13瀛?7.5%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
| 194-16861 | SuperSep Phos-tag鈩?nbsp;(50 渭mol/L), 17.5%, 17 well Phos-tag 17瀛?7.5%棰勫埗鑳迭/td> | 5鍧桙/td> | - |
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